Angiopoietin-2 confers Atheroprotection in apoE-/- mice by inhibiting LDL oxidation via nitric oxide


Atherosclerosis is promoted by a combination of hypercholesterolemia and vascular inflammation. The function of Angiopoietin (Ang)-2, a key regulator of angiogenesis, in the maintenance of large vessels is unknown. A single systemic administration of Ang-2 adenovirus (AdAng-2) to apoE-/- mice fed a Western diet significantly reduced atherosclerotic lesion size 8 40%) and oxidized LDL and macrophage content of the plaques. These beneficial effects were abolished by the inhibition of nitric oxide synthase (NOS). In endothelial cells, endothelial NOS activation per se inhibited LDL oxidation and Ang-2 stimulated NO release in a Tie2-dependent manner to decrease LDL oxidation. These findings demonstrate a novel atheroprotective role for Ang-2 when endothelial cell function is compromised and suggest that growth factors, which stimulate NO release without inducing inflammation, could offer atheroprotection.

Publication DOI:
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Health & Life Sciences > Aston Medical School
Additional Information: © 2009 American Heart Association, Inc.
Uncontrolled Keywords: angiopoietin-2,atherosclerosis,endothelial cells,LDL cholesterol,nitric oxide,nitric oxide synthases,Physiology,Cardiology and Cardiovascular Medicine
Publication ISSN: 1524-4571
Last Modified: 26 Feb 2024 08:12
Date Deposited: 06 Oct 2014 10:55
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://circres. ... ent/104/12/1333 (Publisher URL)
PURE Output Type: Article
Published Date: 2009-06-19
Authors: Ahmed, Asif (ORCID Profile 0000-0002-8755-8546)
Fujisawa, Takeshi
Niu, Xi-Lin
Ahmad, Shakil (ORCID Profile 0000-0002-9294-0475)
Al-Ani, Bahjat
Chudasama, Kunal
Abbas, Allyah
Potluri, Rahul
Bhandari, Vineet
Findley, Clarence M.
Lam, Gregory K.W.
Huang, Jianhua
Hewett, Peter W.
Cudmore, Melissa J.
Kontos, Christopher D.



Version: Accepted Version

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