Developing solid particulate vaccine adjuvants:surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity


This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

Publication DOI:
Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences
College of Health & Life Sciences > Chronic and Communicable Conditions
Additional Information: This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no. 460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.
Uncontrolled Keywords: adjuvant,DDA,ESEM,microspheres,PLGA,subunit vaccine
Publication ISSN: 1875-5704
Last Modified: 15 Jul 2024 07:09
Date Deposited: 19 Aug 2014 08:50
Full Text Link: http://benthams ... rticleID=110594
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2013
Published Online Date: 2013-02-04
Authors: Kirby, Daniel J. (ORCID Profile 0000-0002-0878-2620)
Kaur, Randip
Agger, Else M.
Andersen, Peter
Bramwell, Vincent W.
Perrie, Yvonne



Version: Accepted Version

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