Kirby, Daniel J., Kaur, Randip, Agger, Else M., Andersen, Peter, Bramwell, Vincent W. and Perrie, Yvonne (2013). Developing solid particulate vaccine adjuvants:surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity. Current Drug Delivery, 11 (6), pp. 268-278.
Abstract
This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.
Publication DOI: | https://doi.org/10.2174/1567201811310030003 |
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Divisions: | College of Health & Life Sciences > Aston Pharmacy School College of Health & Life Sciences College of Health & Life Sciences > Chronic and Communicable Conditions |
Additional Information: | This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no. 460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission. |
Uncontrolled Keywords: | adjuvant,DDA,ESEM,microspheres,PLGA,subunit vaccine |
Publication ISSN: | 1875-5704 |
Last Modified: | 30 Sep 2024 10:13 |
Date Deposited: | 19 Aug 2014 08:50 |
Full Text Link: |
http://benthams ... rticleID=110594 |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2013 |
Published Online Date: | 2013-02-04 |
Authors: |
Kirby, Daniel J.
(
0000-0002-0878-2620)
Kaur, Randip Agger, Else M. Andersen, Peter Bramwell, Vincent W. Perrie, Yvonne |