TATPred:a Bayesian method for the identification of twin arginine translocation pathway signal sequences

Abstract

The twin arginine translocation (TAT) system ferries folded proteins across the bacterial membrane. Proteins are directed into this system by the TAT signal peptide present at the amino terminus of the precursor protein, which contains the twin arginine residues that give the system its name. There are currently only two computational methods for the prediction of TAT translocated proteins from sequence. Both methods have limitations that make the creation of a new algorithm for TAT-translocated protein prediction desirable. We have developed TATPred, a new sequence-model method, based on a Nave-Bayesian network, for the prediction of TAT signal peptides. In this approach, a comprehensive range of models was tested to identify the most reliable and robust predictor. The best model comprised 12 residues: three residues prior to the twin arginines and the seven residues that follow them. We found a prediction sensitivity of 0.979 and a specificity of 0.942.

Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences
Additional Information: © 2006 Biomedical Informatics Publishing Group
Uncontrolled Keywords: twin arginine motif,Bayesian networks ,TAT translocation,signal sequence,vaccine
Publication ISSN: 0973-2063
Full Text Link:
Related URLs: http://www.bioi ... net/001/001.htm (Publisher URL)
PURE Output Type: Article
Published Date: 2006-07
Authors: Taylor, Paul D.
Toseland, Christopher P.
Attwood, Teresa K.
Flower, Darren R. (ORCID Profile 0000-0002-8542-7067)

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