Investigation of the Potential Antibacterial Activity of Buddlja Madagascariensis Extracts

Abstract

Evidence suggests that Buddlja Madagascariensis extracts have antibacterial activity and the leaves can be used to cure asthma, coughs and bronchitis. The present investigation is aimed at finding the activity present in Buddlja Madagascariensis against Tuberculosis and identifying the active compounds. Experiments were initiated to assess this possibility using E. coli (DC2), S. aureus (NCTC 6571) and M. fortuitum (NCTC 10394), a model for tuberculosis. The methanol extracts showed some activity against M. fortuitum. The compounds in extraction were separated and purified by HPLC. A series of fractions was achieved. Some of them again showed activity. The fractions then were characterised by mass spectrometry. On the other hand, column purification in a molecular imprinting approach was taken in parallel with the separation of the components in the extraction. The effect was assessed by comparing groups of peaks from HPLC, one of which was for the crude extracts while the rest were for the fractions from column purification.