The isolation of gastric mucosal cells and a study of their isoenzyme patterns

Abstract

Preliminary experiments on cell isolation from rat and pig gastric mucosa revealed that the chelating agent, ethylenediaminetetraacetic acid, or the enzyme collagenase were unsuitable as cell releasing agents for stomach tissue from these two species. Pronase was found to be singularly effective for dissociating the tissue and provided a sufficiently high yield of viable cells for further study. A cell isolation procedure was subsequently developed using pronase and mild mechanical action to release all cell types from prepared pig fundic mucosa. Separation of the different cell types found in gastric mucosa was accomplished by centrifuging the mixed cell suspension in a discontinuous Ficoll density gradient.