MicroRNA-155 promotes resolution of hypoxia-inducible factor 1α activity during prolonged hypoxia


The hypoxia-inducible factor (HIF) is a key regulator of the transcriptional response to hypoxia. While the mechanism underpinning HIF activation is well understood, little is known about its resolution. Both the protein and the mRNA levels of HIF-1a (but not HIF-2a) were decreased in intestinal epithelial cells exposed to prolonged hypoxia. Coincident with this, microRNA (miRNA) array analysis revealed multiple hypoxiainducible miRNAs. Among these was miRNA-155 (miR-155), which is predicted to target HIF-1a mRNA. We confirmed the hypoxic upregulation of miR-155 in cultured cells and intestinal tissue from mice exposed to hypoxia. Furthermore, a role for HIF-1a in the induction of miR-155 in hypoxia was suggested by the identification of hypoxia response elements in the miR-155 promoter and confirmed experimentally. Application of miR-155 decreased the HIF-1a mRNA, protein, and transcriptional activity in hypoxia, and neutralization of endogenous miR-155 reversed the resolution of HIF-1a stabilization and activity. Based on these data and a mathematical model of HIF-1a suppression by miR-155, we propose that miR-155 induction contributes to an isoform-specific negative-feedback loop for the resolution of HIF-1a activity in cells exposed to prolonged hypoxia, leading to oscillatory behavior of HIF-1a-dependent transcription.

Publication DOI: https://doi.org/10.1128/MCB.01276-10
Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Publication ISSN: 1098-5549
Last Modified: 26 Feb 2024 08:12
Date Deposited: 30 Sep 2013 08:24
Full Text Link: http://mcb.asm. ... tent/31/19/4087
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2011-10
Published Online Date: 2011-08-01
Authors: Bruning, Ulrike
Cerone, Luca
Neufeld, Zoltan
Fitzpatrick, Susan F.
Cheong, Alex (ORCID Profile 0000-0003-2482-9078)
Scholz, Carsten C.
Simpson, David A.
Leonard, Martin O.
Tambuwala, Murtaza M.
Cummins, Eoin P.
Taylor, Cormac T.



Version: Published Version

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