Digluconate and isopropyl alcohol biocide formulation


Effective surface disinfection is a fundamental infection control strategy within healthcare. This study assessed the antimicrobial efficacy of novel biocide formulations comprising 5% and 2% eucalyptus oil (EO) combined with 2% chlorhexidine digluconate (CHG) and 70% isopropyl alcohol (IPA) contained within a wipe. The efficacy of this novel antimicrobial formulation to remove and eliminate methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Candida albicans from steel surfaces was investigated. Adpression studies of pre-contaminated wipes were also utilised to assess their potential to induce cross-contamination between hard surfaces. Furthermore, the bactericidal nature of the EO-formulation was established in addition to time-kill. The EO-containing formulations demonstrated bactericidal antimicrobial efficacy against all microorganisms and did not induce surface cross-contamination. There was no significant difference (p < 0.05) between the 5% and 2% EO formulations in their ability to remove microorganisms from steel surfaces, however both significantly (p < 0.05) removed more than the control formulations. Microbial biofilms were eliminated within 10 min (p < 0.05) when exposed to the EO formulations. Our novel EO-formulation demonstrated rapid antimicrobial efficacy for potential disinfection and elimination of microbial biofilms from hard surfaces and may therefore be a useful adjunct to current infection control strategies currently employed within healthcare facilities.

Publication DOI: https://doi.org/10.3390/ijms131114016
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > Chronic and Communicable Conditions
Additional Information: Creative Commons Attribution License
Uncontrolled Keywords: eucalyptus oil,green biocide,biofilm,surface disinfection,chlorhexidine digluconate,healthcare associated infection,Computer Science Applications,Molecular Biology,Catalysis,Inorganic Chemistry,Spectroscopy,Organic Chemistry,Physical and Theoretical Chemistry
Publication ISSN: 1422-0067
Last Modified: 22 Feb 2024 08:07
Date Deposited: 20 May 2013 09:18
Full Text Link: http://www.mdpi ... 067/13/11/14016
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2012-10-30
Authors: Hendry, Emma
Conway, Barbara
Worthington, Tony (ORCID Profile 0000-0002-1906-3357)



Version: Published Version

License: Creative Commons Attribution

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