Overcoming barriers to membrane protein structure determination


After decades of slow progress, the pace of research on membrane protein structures is beginning to quicken thanks to various improvements in technology, including protein engineering and microfocus X-ray diffraction. Here we review these developments and, where possible, highlight generic new approaches to solving membrane protein structures based on recent technological advances. Rational approaches to overcoming the bottlenecks in the field are urgently required as membrane proteins, which typically comprise ~30% of the proteomes of organisms, are dramatically under-represented in the structural database of the Protein Data Bank.

Publication DOI: https://doi.org/10.1038/nbt.1833
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Health & Life Sciences > School of Biosciences
Uncontrolled Keywords: crystallization,protein databases,membrane proteins,protein engineering,protein stability,tertiary protein structure,protein unfolding,recombinant proteins,X-ray diffraction,Applied Microbiology and Biotechnology,Biotechnology,Molecular Medicine,Bioengineering,Biomedical Engineering
Publication ISSN: 1546-1696
Last Modified: 26 Feb 2024 08:11
Date Deposited: 01 May 2013 10:57
Full Text Link: http://www.natu ... l/nbt.1833.html
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2011-04-08
Authors: Bill, Roslyn M. (ORCID Profile 0000-0003-1331-0852)
Henderson, Peter J.F.
Iwata, So
Kunji, Edmund R.S.
Michel, Hartmut
Neutze, Richard
Newstead, Simon
Poolman, Bert
Tate, Christopher G.
Vogel, Horst



Version: Accepted Version

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