Bill, Roslyn M., Henderson, Peter J.F., Iwata, So, Kunji, Edmund R.S., Michel, Hartmut, Neutze, Richard, Newstead, Simon, Poolman, Bert, Tate, Christopher G. and Vogel, Horst (2011). Overcoming barriers to membrane protein structure determination. Nature Biotechnology, 29 (4), pp. 335-340.
Abstract
After decades of slow progress, the pace of research on membrane protein structures is beginning to quicken thanks to various improvements in technology, including protein engineering and microfocus X-ray diffraction. Here we review these developments and, where possible, highlight generic new approaches to solving membrane protein structures based on recent technological advances. Rational approaches to overcoming the bottlenecks in the field are urgently required as membrane proteins, which typically comprise ~30% of the proteomes of organisms, are dramatically under-represented in the structural database of the Protein Data Bank.
Publication DOI: | https://doi.org/10.1038/nbt.1833 |
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Divisions: | College of Health & Life Sciences College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine College of Health & Life Sciences > School of Biosciences |
Uncontrolled Keywords: | crystallization,protein databases,membrane proteins,protein engineering,protein stability,tertiary protein structure,protein unfolding,recombinant proteins,X-ray diffraction,Applied Microbiology and Biotechnology,Biotechnology,Molecular Medicine,Bioengineering,Biomedical Engineering |
Publication ISSN: | 1546-1696 |
Last Modified: | 30 Sep 2024 10:01 |
Date Deposited: | 01 May 2013 10:57 |
Full Text Link: |
http://www.natu ... l/nbt.1833.html |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2011-04-08 |
Authors: |
Bill, Roslyn M.
(
0000-0003-1331-0852)
Henderson, Peter J.F. Iwata, So Kunji, Edmund R.S. Michel, Hartmut Neutze, Richard Newstead, Simon Poolman, Bert Tate, Christopher G. Vogel, Horst |