Improving the repeatability of heterochromatic flicker photometry for measurement of macular pigment optical density


Background: Heterochromatic flicker photometry (HFP) is a psychophysical technique used to measure macular pigment optical density (MPOD). We used the MPS 9000 (MPS) HFP device. Our aim was to determine if the repeatability of the MPS could be improved to make it more suitable for monitoring MPOD over time. Methods: Intra-session repeatability was assessed in 25 participants (aged 20-50 years). The resulting data was explored in detail, e.g., by examining the effect of removal and adjustment of data with less than optimal quality parameters. A protocol was developed for improved overall reliability, which was then tested in terms of inter-session repeatability in a separate group of 27 participants (aged 19-52 years). Results: Removal and adjustment of data reduced the intra-session coefficient of repeatability (CR) by 0.04, on average, and the mean individual standard deviation by 0.004. Raw data observation offered further insight into ways of improving repeatability. The proposed protocol resulted in an inter-session CR of 0.08. Conclusions: Removal and adjustment of less than optimal data improved repeatability, and is therefore recommended. To further improve repeatability, in brief we propose that each patient perform each part of the test twice, and a third time where necessary (described in detail by the protocol). Doing so will make the MPS more useful in research and clinical settings. © 2012 Springer-Verlag.

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Divisions: College of Health & Life Sciences > School of Optometry > Optometry
College of Health & Life Sciences > School of Optometry > Optometry & Vision Science Research Group (OVSRG)
College of Health & Life Sciences
College of Health & Life Sciences > School of Optometry > Vision, Hearing and Language
Additional Information: The original publication is available at
Uncontrolled Keywords: age-related macular degeneration,heterochromatic flicker photometry,macular pigment,macular pigment optical density,Sensory Systems,Cellular and Molecular Neuroscience,Ophthalmology
Publication ISSN: 1435-702X
Last Modified: 18 Jun 2024 07:10
Date Deposited: 17 Jan 2013 13:36
Full Text Link: http://link.spr ... 0417-012-2127-0
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2013-03-01
Published Online Date: 2012-08-17
Authors: Howells, Olivia
Eperjesi, Frank (ORCID Profile 0000-0003-4358-0095)
Bartlett, Hannah (ORCID Profile 0000-0002-7531-6902)



Version: Draft Version

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