Identifying a novel functional domain within the p115 tethering factor required for Golgi ribbon assembly and membrane trafficking


The tethering factor p115 has been shown to facilitate Golgi biogenesis and membrane traffic in cells in culture. However, the role of p115 within an intact animal is largely unknown. Here, we document that RNAi-mediated depletion of p115 in C. elegans causes accumulation of the yolk protein (YP170) in body cavity and the retention of the yolk receptor RME-2 in the ER and the Golgi within oocytes.Structure-function analyses of p115 have identified two homology (H1-2) regions within the N-terminal globular head and the coiled-coil 1 (CC1) domain as essential for p115 function. We identify a novel C-terminal domain of p115 as necessary for Golgi ribbon formation and cargo trafficking. We show that p115 mutants lacking the fourth CC domain (CC4) act in a dominant negative manner to disrupt Golgi and prevent cargo trafficking in cells containing endogenous p115. Furthermore, using RNAi-mediated "replacement" strategy we show that CC4 is necessary for Golgi ribbon formation and membrane trafficking in cells depleted of endogenous p115.p115 has been shown to bind a subset of ER-Golgi SNAREs through CC1 and CC4 domains (Shorter et al., 2002). Our findings show that CC4 is required for p115 function and suggest that both the CC1 and the CC4 SNARE-binding motifs may participate in p115-mediated membrane tethering.

Publication DOI:
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Health & Life Sciences > School of Biosciences
Additional Information: © 2012. Published by The Company of Biologists Ltd
Uncontrolled Keywords: Cell Biology
Publication ISSN: 1477-9137
Last Modified: 05 Jun 2024 07:09
Date Deposited: 10 Aug 2012 11:57
Full Text Link: https://www.ncb ... les/PMC4450726/
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://jcs.biol ... tent/125/8/1896 (Publisher URL)
PURE Output Type: Article
Published Date: 2012-04-15
Published Online Date: 2012-02-10
Authors: Grabski, Robert
Balklava, Zita (ORCID Profile 0000-0001-9039-9710)
Wyrozumska, Paulina
Szul, Tomasz
Brandon, Elizabeth
Alvarez, Cecilia
Holloway, Zoe G.
Sztul, Elizabeth



Version: Published Version

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