Understanding the yeast host cell response to recombinant membrane protein production


Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples for further research underpins the pharmaceutical industry's strategy for developing new drugs. This is typically achieved by synthesizing a protein of interest in host cells that can be cultured on a large scale, allowing the isolation of the pure protein in quantities much higher than those found in the protein's native source. Yeast is a popular host as it is a eukaryote with similar synthetic machinery to that of the native human source cells of many proteins of interest, while also being quick, easy and cheap to grow and process. Even in these cells, the production of human membrane proteins can be plagued by low functional yields; we wish to understand why. We have identified molecular mechanisms and culture parameters underpinning high yields and have consolidated our findings to engineer improved yeast host strains. By relieving the bottlenecks to recombinant membrane protein production in yeast, we aim to contribute to the drug discovery pipeline, while providing insight into translational processes.

Publication DOI: https://doi.org/10.1042/BST0390719
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: This research was originally published in Biochemical Society transactions. Bawa, Z, Bland, CE, Bonander, N, Bora, N, Cartwright, SP, Clare, M, Conner, MT, Darby, RA, Dilworth, MV, Holmes, WJ, Jamshad, M, Routledge, SJ, Gross, SR & Bill, RM 2011, 'Understanding the yeast host cell response to recombinant membrane protein production', Biochemical Society transactions, vol 39, no. 3, pp. 719-723. © the Biochemical Society.
Uncontrolled Keywords: bioengineering,humans,membrane proteins,recombinant proteins,saccharomyces cerevisiae,yeasts,Biochemistry
Publication ISSN: 1470-8752
Last Modified: 03 Jun 2024 07:10
Date Deposited: 22 Jun 2012 12:08
Full Text Link: http://www.bioc ... /bst0390719.htm
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2011-06
Authors: Bawa, Zharain
Bland, Charlotte E.
Bonander, Nicklas
Bora, Nagamani
Cartwright, Stephanie P.
Clare, Michelle
Conner, Matthew T.
Darby, Richard A.J.
Dilworth, Marvin V
Holmes, William J.
Jamshad, Mohammed
Routledge, Sarah J.
Gross, Stephane R. (ORCID Profile 0000-0002-0867-8866)
Bill, Roslyn M. (ORCID Profile 0000-0003-1331-0852)



Version: Accepted Version

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