Synthesis of Peptide Nucleic Acid (PNA) and Entrapment of PNA into Liposomes

Abstract

Peptide Nucleic Acid (PNA) is a synthetic pseudopeptide nucleic acid analogue in which the deoxyribose phosphodiester backbone of DNA is replaced by a pseudo-peptide composed of N-(2 aminoethyl)glycine units, resulting in an achiral and uncharged DNA mimic. PNA is chemically stable and resistant to enzymatic cleavage and with high binding affinity and specificity to nucleic acids. Therefore PNAs are very strong candidates as effective antisense or antigene agents. They have shown both antigene and antisense activities against target genes and transcripts, respectively. PNAs are also very useful biomolecular tools. Unfortunately, for certain applications of PNA, there are some severe drawbacks such as its poor water solubility and poor cellular uptake. Fast progress in the exploration of PNA as an experimental and therapeutic regulator of gene expression has been hampered by these drawbacks. Several transfection protocols for PNA have now been studied, such as microinjection, electroporation and co-transfection with DNA. Among the different delivery strategies, liposomes are a potential way to delivery PNA into cells since liposomes can protect various molecules from the external medium and can deliver molecules directly into cells. Moreover, it is also possible to specifically target liposomes by coupling proteins or antibodies to their surface. In this project PNA oligomers were synthesized and labeled with fluorescein. These oligomers were entrapped into the liposome and from the results, we can draw a conclusion that the method of dehydration-rehydration is encouraging method for the preparation of PNA-liposomal delivery systems which can offer high PNA loading, mild preparation methods and the potential to optimise PNA release.

Publication DOI: https://doi.org/10.48780/publications.aston.ac.uk.00011024
Divisions: College of Health & Life Sciences > School of Biosciences
Additional Information: Copyright © Peng He, 2004. Peng He asserts their moral right to be identified as the author of this thesis. This copy of the thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with its author and that no quotation from the thesis and no information derived from it may be published without appropriate permission or acknowledgement. If you have discovered material in Aston Publications Explorer which is unlawful e.g. breaches copyright, (either yours or that of a third party) or any other law, including but not limited to those relating to patent, trademark, confidentiality, data protection, obscenity, defamation, libel, then please read our Takedown Policy and contact the service immediately.
Institution: Aston University
Uncontrolled Keywords: peptide nucleic acid,entrapment,liposomes
Last Modified: 14 May 2025 13:26
Date Deposited: 21 Dec 2010 13:49
Completed Date: 2004-09
Authors: He, Peng

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