The Separation of Fructose from Carbohydrate Mixtures by Chromatographic Techniques

Abstract

Chromatography is used extensively in the chemical industry as an analytical tool but in the last decade has been adopted for large scale separation of organic mixtures. In this thesis a review is given of the general chromatographic theory, the factors affecting the performance of chromatographic columns and aspects of scale up of the chromatographic process. Also included is a review of methods available for the production of high fructose syrup and the processes in operation for enriching the fructose content in the syrup. Various industrial scale chromatographic processes, currently in operation, have been outlined. The description and operation of a liquid-solid chromatographic refiner (SCCR) is described. Counter current operation was simulated by sequencing a system of inlet and outlet port functions around ten, 108 mm internal diameter x 650 mm long, stainless steel columns packed with a calcium charged zerolit SRC 14 resin (150-300 tm size range). This equipment was surrounded by a constant temperature enclosure...