Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing

Gomes, Cláudia Sg, Fashina, Adedayo, Fernández-castané, Alfred, Overton, Timothy W, Hobley, Timothy J, Theodosiou, Eirini and Thomas, Owen Rt (2018). Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing. Journal of Chemical Technology and Biotechnology ,

Abstract

BACKGROUND The extraction of biopharmaceuticals from plasma and serum often employs overly complicated antiquated procedures, that can inflict serious damage on especially prone protein targets and which afford low purification power and overall yields. Here we describe systematic development of a high-gradient magnetic fishing process for recovery of immunoglobulins from unclarified antiserum. RESULTS Non-porous superparamagnetic particles were transformed into hydrophobic-charge induction adsorbents and then used to recover immunoglobulins from rabbit antiserum feedstocks. Comprehensive characterisation tests conducted with variously diluted clarified antiserum on a magnetic rack revealed that immunoglobulin binding was rapid (equilibrium reached in <45 s), strong (Kd <0.1 mg mL-1), of high capacity (Qmax = 214 mg g-1), and pH and ionic strength dependent. In a high-gradient magnetic fishing process conducted with the same adsorbent, and a conventional ‘magnetic filter + recycle loop’ arrangement, >72% of the immunoglobulin present in an unclarified antiserum feed was recovered in 0.5 h in >3-fold purified form. CONCLUSIONS Fast magnetic particle based capture of antibodies from an unclarified high-titre feed has been demonstrated. Efficient product recovery from ultra-high titre bioprocess liquors by high-gradient magnetic fishing requires that improved magnetic adsorbents displaying high selectivity, ultra-high capacity and operational robustness are used with 'state-of-the-art’ rotor-stator magnetic separators.

Publication DOI: https://doi.org/10.1002/jctb.5599
Divisions: Engineering & Applied Sciences
Engineering & Applied Sciences > Aston Institute of Materials Research (AIMR)
Engineering & Applied Sciences > European Bioenergy Research Institute (EBRI)
Additional Information: Funding: Biotechnology & Biological Sciences Research Council (BBSRC) and the Danish Council forS trategic Research( DetStrategiskeForskningsråd).
Uncontrolled Keywords: direct capture,polyclonal & monoclonal antibodies,4-mercaptoethylpyridine,serum & plasma,complex unclarified bioprocess liquors
Full Text Link: http://doi.wile ... .1002/jctb.5599
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Published Date: 2018-02-02
Authors: Gomes, Cláudia Sg
Fashina, Adedayo
Fernández-castané, Alfred ( 0000-0002-2572-7797)
Overton, Timothy W
Hobley, Timothy J
Theodosiou, Eirini ( 0000-0001-7068-4434)
Thomas, Owen Rt

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