RECQL5 controls transcript elongation and suppresses genome instability associated with transcription stress

Saponaro, Marco, Kantidakis, Theodoros, Mitter, Richard, Kelly, Gavin P., Heron, Mark, Williams, Hannah, Söding, Johannes, Stewart, Aengus and Svejstrup, Jesper Q. (2014). RECQL5 controls transcript elongation and suppresses genome instability associated with transcription stress. Cell, 157 (5), pp. 1037-1049.

Abstract

RECQL5 is the sole member of the RECQ family of helicases associated with RNA polymerase II (RNAPII). We now show that RECQL5 is a general elongation factor that is important for preserving genome stability during transcription. Depletion or overexpression of RECQL5 results in corresponding shifts in the genome-wide RNAPII density profile. Elongation is particularly affected, with RECQL5 depletion causing a striking increase in the average rate, concurrent with increased stalling, pausing, arrest, and/or backtracking (transcription stress). RECQL5 therefore controls the movement of RNAPII across genes. Loss of RECQL5 also results in the loss or gain of genomic regions, with the breakpoints of lost regions located in genes and common fragile sites. The chromosomal breakpoints overlap with areas of elevated transcription stress, suggesting that RECQL5 suppresses such stress and its detrimental effects, and thereby prevents genome instability in the transcribed region of genes.

Publication DOI: https://doi.org/10.1016/j.cell.2014.03.048
Divisions: Aston Medical School
Additional Information: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
Uncontrolled Keywords: Biochemistry, Genetics and Molecular Biology(all)
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2014-05-22
Authors: Saponaro, Marco
Kantidakis, Theodoros ( 0000-0002-3515-5235)
Mitter, Richard
Kelly, Gavin P.
Heron, Mark
Williams, Hannah
Söding, Johannes
Stewart, Aengus
Svejstrup, Jesper Q.

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