Production of membrane proteins in yeast

Jamshad, Mohammed, Darby, Richard A. J., Grgic, Ljuban and Bill, Roslyn M. (2006). Production of membrane proteins in yeast. Biochemistry and Cell Biology, 84 (6), p. 1069.


Background Yeast is an important and versatile organism for studying membrane proteins. It is easy to cultivate and can perform higher eukaryote-like post-translational modifications. S. cerevisiae has a fully-sequenced genome and there are several collections of deletion strains available, whilst P. pastoris can produce very high cell densities (230 g/l). Results We have used both S. cerevisiae and P. pastoris to over-produce the following His6 and His10 carboxyl terminal fused membrane proteins. CD81 – 26 kDa tetraspanin protein (TAPA-1) that may play an important role in the regulation of lymphoma cell growth and may also act as the viral receptor for Hepatitis C-Virus. CD82 – 30 kDa tetraspanin protein that associates with CD4 or CD8 cells and delivers co-stimulatory signals for the TCR/CD3 pathway. MC4R – 37 kDa seven transmembrane G-protein coupled receptor, present on neurons in the hypothalamus region of the brain and predicted to have a role in the feast or fast signalling pathway. Adt2p – 34 kDa six transmembrane protein that catalyses the exchange of ADP and ATP across the yeast mitochondrial inner membrane. Conclusion We show that yeasts are flexible production organisms for a range of different membrane proteins. The yields are such that future structure-activity relationship studies can be initiated via reconstitution, crystallization for X-ray diffraction or NMR experiments.

Publication DOI:
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Cellular and Molecular Biomedicine
Additional Information: © Darby et al; licensee BioMed Central Ltd. 2006
Uncontrolled Keywords: yeast,membrane proteins,eukaryote-like post-translational modifications,S. cerevisiae,fully-sequenced genom,P. pastoris,cell densities,lymphoma cell growth,viral receptor,Hepatitis C-Virus,CD82 – 30 kDa tetraspanin protein,CD4 cel,MC4R – 37 kDa seven transmembrane G-protein coupled receptor,neurons,hypothalamus,brain,Adt2p – 34 kDa six transmembrane protein,yeast mitochondrial inner membrane,reconstitution,crystallization for X-ray diffraction,NMR experiments
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Related URLs: http://www.micr ... ontent/5/S1/P38 (Publisher URL)
Published Date: 2006-12
Authors: Jamshad, Mohammed
Darby, Richard A. J.
Grgic, Ljuban
Bill, Roslyn M. ( 0000-0003-1331-0852)



Version: Published Version

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