Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks

Abstract

Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing defined in vitro human neural networks for application in influencing neurite guidance and complex network activity.

Publication DOI: https://doi.org/10.1039/c9lc01209e
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences > Biosciences
Engineering & Applied Sciences > Aston Institute of Photonics Technology
Engineering & Applied Sciences
Life & Health Sciences > Chronic and Communicable Conditions
Life & Health Sciences
Life & Health Sciences > Clinical and Systems Neuroscience
Additional Information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Funding: This project has received funding from the European Union's Horizon 2020 research and innovation programme under the grant agreement MESOBRAIN No 713140. EUR and BNC acknowledge further financial support from the European Union's Horizon 2020 programme under the grant agreement SCAFFOLD-NEEDS No. 851734.
Uncontrolled Keywords: Bioengineering,Biochemistry,Chemistry(all),Biomedical Engineering
Full Text Link:
Related URLs: https://pubs.rs ... 9E#!divAbstract (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2020-05-19
Published Online Date: 2020-04-21
Accepted Date: 2020-04-12
Authors: Crowe, J A
El-Tamer, A
Nagel, D
Koroleva, A V
Madrid-Wolff, J
Olarte, O E
Sokolovsky, S ( 0000-0001-7445-7204)
Estevez-Priego, E
Ludl, A-A
Soriano, J
Loza-Alvarez, P
Chichkov, B N
Hill, E J ( 0000-0002-9419-1500)
Parri, H R ( 0000-0002-1412-2688)
Rafailov, E U ( 0000-0002-4152-0120)

Download

[img]

Version: Published Version

License: Creative Commons Attribution

| Preview

Export / Share Citation


Statistics

Additional statistics for this record