DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre

Abstract

In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.

Publication DOI: https://doi.org/10.1117/12.886070
Divisions: Engineering & Applied Sciences > Electrical, Electronic & Power Engineering
Additional Information: Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Event Title: 21st International Conference on Optical Fiber Sensors
Event Type: Other
Event Dates: 2011-05-15 - 2011-05-19
Uncontrolled Keywords: biosensing,DNA,fluorescence,hollow core,hydrogel,Photonic crystal fibres,silanisation,Electronic, Optical and Magnetic Materials,Condensed Matter Physics,Computer Science Applications,Applied Mathematics,Electrical and Electronic Engineering
ISBN: 9780819482464
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Conference contribution
Published Date: 2011-06-09
Authors: Rutowska, Monika
Lu, Jiadi
Garcia-Gunning, Fatima C.
Ellis, Andrew D. ( 0000-0002-0417-0547)

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