Disordered protein-graphene oxide co-assembly and supramolecular biofabrication of functional fluidic devices

Abstract

Supramolecular chemistry offers an exciting opportunity to assemble materials with molecular precision. However, there remains an unmet need to turn molecular self-assembly into functional materials and devices. Harnessing the inherent properties of both disordered proteins and graphene oxide (GO), we report a disordered protein-GO co-assembling system that through a diffusion-reaction process and disorder-to-order transitions generates hierarchically organized materials that exhibit high stability and access to non-equilibrium on demand. We use experimental approaches and molecular dynamics simulations to describe the underlying molecular mechanism of formation and establish key rules for its design and regulation. Through rapid prototyping techniques, we demonstrate the system's capacity to be controlled with spatio-temporal precision into well-defined capillary-like fluidic microstructures with a high level of biocompatibility and, importantly, the capacity to withstand flow. Our study presents an innovative approach to transform rational supramolecular design into functional engineering with potential widespread use in microfluidic systems and organ-on-a-chip platforms.

Publication DOI: https://doi.org/10.1038/s41467-020-14716-z
Divisions: Engineering & Applied Sciences > Mathematics
Additional Information: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Uncontrolled Keywords: Chemistry(all),Biochemistry, Genetics and Molecular Biology(all),Physics and Astronomy(all)
Full Text Link:
Related URLs: https://www.nat ... 467-020-14716-z (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2020-03-04
Accepted Date: 2020-01-24
Authors: Wu, Yuanhao
Okesola, Babatunde O
Xu, Jing
Korotkin, Ivan
Berardo, Alice
Corridori, Ilaria
di Brocchetti, Francesco Luigi Pellerej
Kanczler, Janos
Feng, Jingyu
Li, Weiqi
Shi, Yejiao
Farafonov, Vladimir
Wang, Yiqiang
Thompson, Rebecca F
Titirici, Maria-Magdalena
Nerukh, Dmitry ( 0000-0001-9005-9919)
Karabasov, Sergey
Oreffo, Richard O C
Carlos Rodriguez-Cabello, Jose
Vozzi, Giovanni
Azevedo, Helena S
Pugno, Nicola M
Wang, Wen
Mata, Alvaro

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