The light-oxygen effect in biological cells enhanced by highly localized surface plasmon-polaritons

Abstract

Here at the first time we suggested that the surface plasmon-polariton phenomenon which it is well described in metallic nanostructures could also be used for explanation of the unexpectedly strong oxidative effects of the low-intensity laser irradiation in living matters (cells, tissues, organism). We demonstrated that the narrow-band laser emitting at 1265 nm could generate significant amount of the reactive oxygen species (ROS) in both HCT116 and CHO-K1 cell cultures. Such cellular ROS effects could be explained through the generation of highly localized plasmon-polaritons on the surface of mitochondrial crista. Our experimental conditions, the low-intensity irradiation, the narrow spectrum band (<4 nm) of the laser and comparably small size bio-structures (~10 μm) were shown to be sufficient for the plasmon-polariton generation and strong laser field confinement enabling the oxidative stress observed.

Publication DOI: https://doi.org/10.1038/s41598-019-54905-5
Divisions: Engineering & Applied Sciences > Aston Institute of Photonics Technology
Engineering & Applied Sciences
Additional Information: © The Author(s) 2019. Open Access - This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Funding: Russian Foundation for Basic Research (RFBR) (#18-29-19101 (I.Z., D.S.)). Russian Science Foundation (RSF) (#18-12-00457 (A.F., I.Z., D.S.). EU H2020 FET research and innovation program MESO-BRAIN (#713140 (E.R., S.S.)). Ministry of Science and Higher Education of the Russian Federation through the assignment of 2019 year for Scientific-Manufacturing Complex “Technological Centre”.Ministry of Education and Science of Russian Federation (Grant № 12.1223.2017/AP to S.S. and E.R.).
Uncontrolled Keywords: Biological physics,Biophotonics,Biophysics,Lasers, LEDs and light sources,Micro-optics,General
Full Text Link:
Related URLs: http://www.natu ... 598-019-54905-5 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2019-12-05
Accepted Date: 2019-11-12
Authors: Khokhlova, Anna
Zolotovskii, Igor
Sokolovski, Sergei (ORCID Profile 0000-0001-7445-7204)
Saenko, Yury
Rafailov, Edik (ORCID Profile 0000-0002-4152-0120)
Stoliarov, Dmitrii
Pogodina, Evgenia
Svetukhin, Vyacheslav
Sibirny, Vladimir
Fotiadi, Andrei

Download

[img]

Version: Published Version

License: Creative Commons Attribution

| Preview

Export / Share Citation


Statistics

Additional statistics for this record