Bioprocess forces and their impact on cell behavior:implications for bone regeneration therapy

Abstract

Bioprocess forces such as shear stress experienced during routine cell culture are considered to be harmful to cells. However, the impact of physical forces on cell behavior is an area of growing interest within the tissue engineering community, and it is widely acknowledged that mechanical stimulation including shear stress can enhance osteogenic differentiation. This paper considers the effects of bioprocess shear stress on cell responses such as survival and proliferation in several contexts, including suspension-adapted cells used for recombinant protein and monoclonal antibody manufacture, adherent cells for therapy in suspension, and adherent cells attached to their growth substrates. The enhanced osteogenic differentiation that fluid flow shear stress is widely found to induce is discussed, along with the tissue engineering of mineralized tissue using perfusion bioreactors. Recent evidence that bioprocess forces produced during capillary transfer or pipetting of cell suspensions can enhance osteogenic responses is also discussed.

Publication DOI: https://doi.org/10.4061/2011/620247
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Additional Information: Copyright © 2011 David Brindley et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
PURE Output Type: Article
Published Date: 2011
Authors: Brindley, David
Moorthy, Kishaani
Lee, Jae-Ho
Mason, Chris
Kim, Hae-Won
Wall, Ivan ( 0000-0001-6294-8348)

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