Muscle-derived precursor cells isolated on the basis of differential adhesion properties respond differently to capillary flow

Abstract

Capillary shear stress can improve osteogenic differentiation in muscle-derived precursor cells (MDPCs). This has implications for large-scale bioprocessing of cell therapies where capillary transfer is needed. The recovery, viability, and osteogenic differentiation potential of two subsets of MDPCs, early-adherent pre-plate 1 (PP1) and late-adherent PP3 populations, have been examined: PP1 MDPCs produced a greater degree of osteogenic differentiation than PP3 MDPCs, quantified by Alizarin Red S staining intensity (P < 0.05). For both cell populations, capillary flow-induced significant increases in Alizarin Red S staining (P < 0.05). However, PP1 cells were more susceptible to capillary flow-induced damage than PP3 cells and this was dependent on duration of exposure. Overall, results indicate that different cell subsets, even from within a single tissue, can respond variably to capillary shear stress, necessitating its precise monitoring and control.

Publication DOI: https://doi.org/10.1007/s10529-011-0570-3
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Additional Information: © Springer Science+Business Media B.V. 2011. The final publication is available at Springer via http://dx.doi.org/10.1007/s10529-011-0570-3
Uncontrolled Keywords: Differential adhesion,Muscle-derived precursor cells,Osteogenic differentiation,Shear stress,Stem cells,Biotechnology
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://link.sp ... 0529-011-0570-3 (Publisher URL)
PURE Output Type: Article
Published Date: 2011-07-01
Authors: Patel, Minal
Mulhall, Hayley
Al-Quatani, Khalid
Lewis, Mark
Wall, Ivan ( 0000-0001-6294-8348)

Download

[img]

Version: Accepted Version

| Preview

Export / Share Citation


Statistics

Additional statistics for this record