A closer look at neuron interaction with track-etched microporous membranes

Abstract

Microporous membranes support the growth of neurites into and through micro-channels, providing a different type of neural growth platform to conventional dish cultures. Microporous membranes are used to support various types of culture, however, the role of pore diameter in relation to neurite growth through the membrane has not been well characterised. In this study, the human cell line (SH-SY5Y) was differentiated into neuron-like cells and cultured on track-etched microporous membranes with pore and channel diameters selected to accommodate neurite width (0.8 µm to 5 µm). Whilst neurites extended through all pore diameters, the extent of neurite coverage on the non-seeded side of the membranes after 5 days in culture was found to be directly proportional to channel diameter. Neurite growth through membrane pores reduced significantly when neural cultures were non-confluent. Scanning electron microscopy revealed that neurites bridged pores and circumnavigated pore edges – such that the overall likelihood of a neurite entering a pore channel was decreased. These findings highlight the role of pore diameter, cell sheet confluence and contact guidance in directing neurite growth through pores and may be useful in applications that seek to use physical substrates to maintain separate neural populations whilst permitting neurite contact between cultures.

Publication DOI: https://doi.org/10.1038/s41598-018-33710-6
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences > Cell & Tissue Biomedical Research
Life & Health Sciences
Life & Health Sciences > Chronic and Communicable Conditions
Life & Health Sciences > Clinical and Systems Neuroscience
Life & Health Sciences > Pharmacy
Life & Health Sciences > Cellular and Molecular Biomedicine
Additional Information: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Full Text Link:
Related URLs: http://www.natu ... 598-018-33710-6 (Publisher URL)
PURE Output Type: Article
Published Date: 2018-10-19
Accepted Date: 2018-10-03
Authors: George, Julian H.
Nagel, David
Waller, Sharlayne
Hill, Eric ( 0000-0002-9419-1500)
Parri, H. Rhein ( 0000-0002-1412-2688)
Coleman, Michael D.
Cui, Zhanfeng
Ye, Hua

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