Epithelial transport and deamidation of gliadin peptides:a role for coeliac disease patient immunoglobulin A


In coeliac disease, the intake of dietary gluten induces small-bowel mucosal damage and the production of immunoglobulin (Ig)A class autoantibodies against transglutaminase 2 (TG2). We examined the effect of coeliac patient IgA on the apical-to-basal passage of gluten-derived gliadin peptides p31-43 and p57-68 in intestinal epithelial cells. We demonstrate that coeliac IgA enhances the passage of gliadin peptides, which could be abolished by inhibition of TG2 enzymatic activity. Moreover, we also found that both the apical and the basal cell culture media containing the immunogenic gliadin peptides were able to induce the proliferation of deamidation-dependent coeliac patient-derived T cells even in the absence of exogenous TG2. Our results suggest that coeliac patient IgA could play a role in the transepithelial passage of gliadin peptides, a process during which they might be deamidated.

Publication DOI: https://doi.org/10.1111/j.1365-2249.2010.04317.x
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Life & Health Sciences > Chronic and Communicable Conditions
Life & Health Sciences > Cellular and Molecular Biomedicine
Aston University (General)
Additional Information: © 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology.
Uncontrolled Keywords: amides,amino acid sequence,autoantibodies,caco-2 cells,celiac disease,cell proliferation,enzyme inhibitors,epithelial cells,GTP-binding proteins,gliadin,humans,immunoglobulin A,small intestine,peptide fragments,protein transport,T-lymphocytes,transglutaminases
Full Text Link: https://www.ncb ... les/PMC3074225/
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://onlineli ... 4317.x/abstract (Publisher URL)
PURE Output Type: Article
Published Date: 2011-04
Published Online Date: 2011-01-01
Authors: Rauhavirta, T.
Qiao, S.-W.
Jiang, Z.
Myrsky, E.
Loponen, J.
Korponay-Szabó, I.R.
Salovaara, H.
Garcia-Horsman, J.A.
Venäläinen, J.
Männistö, P.T.
Collighan, R. ( 0000-0003-0204-5125)
Mongeot, A.
Griffin, M.
Mäki, M.
Kaukinen, K.
Lindfors, K.

Export / Share Citation


Additional statistics for this record