An initial evaluation of gellan gum as a material for tissue engineering applications


Alpha-modified minimum essential medium (αMEM) has been found to cross-link a 1% gellan gum solution, resulting in the formation of a self-supporting hydrogel in 1:1 and 5:1 ratios of polysaccharide: αMEM. Rheological data from temperature sweeps confirm that in addition to orders of magnitude differences in G' between 1% gellan and 1% gellan with αMEM, there is also a 20°C increase in the temperature at which the onset of gelation takes place when αMEM is present. Frequency sweeps confirm the formation of a true gel; mechanical spectra for mixtures of gellan and αMEM clearly demonstrate G' to be independent of frequency. It is possible to immobilize cells within a three-dimensional (3D) gellan matrix that remain viable for up to 21 days in culture by adding a suspension of rat bone marrow cells (rBMC) in αMEM to 1% gellan solution. This extremely simple approach to cell immobilization within 3D constructs, made possible by the fact that gellan solutions cross-link in the presence of millimolar concentrations of cations, poses a very low risk to a cell population immobilized within a gellan matrix and thus indicates the potential of gellan for use as a tissue engineering scaffold. © 2007 Sage Publications.

Publication DOI:
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Life & Health Sciences > Chronic and Communicable Conditions
Uncontrolled Keywords: bone,cell viability,gellan,hydrogel,three-dimensional,Biomedical Engineering,Biomaterials
Full Text Link: https://hal.arc ... 570778/document
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://jba.sage ... /3/241.abstract (Publisher URL)
PURE Output Type: Article
Published Date: 2007-11-16
Published Online Date: 2007-05-10
Authors: Smith, Alan M.
Shelton, Richard M.
Perrie, Yvonne
Harris, Jonathan J.

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