Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells


Nanoscale extracellular vesicles (EVs) including exosomes (50-150 nm membrane particles) have emerged as promising cancer biomarkers due to the carried genetic information about the parental cells. However the sensitive detection of these vesicles remains a challenge. Here we present a label-free electrochemical sensor to measure the EVs secretion levels of hypoxic and normoxic MCF-7 cells. The sensor design includes two consecutive steps; i) Au electrode surface functionalization for anti-CD81 Antibody and ii) EVs capture. The label-free detection of EVs was done via Differential Pulse Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS). The working linear range for the sensor was 102-109 EVs/ml with an LOD 77 EVs/mL and 379 EVs/ml for EIS and DPV based detection. A blood-abundant protein, RhD was used for the selectivity test. In order to assess the performance of the biosensor, the level of EVs secretion by the human breast cancer MCF-7 cell line was compared with enzyme-linked immunosorbent assays (ELISA) and Nanoparticle Tracking Analysis (NTA). Designed label-free electrochemical sensors utilized for quantification of EVs secretion enhancement due to CoCl2-induced hypoxia and 1.23 fold increase with respect to normoxic conditions was found.

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Divisions: Life & Health Sciences
Life & Health Sciences > Cell & Tissue Biomedical Research
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Related URLs: https://www.nat ... 598-018-27203-9 (Publisher URL)
PURE Output Type: Article
Published Date: 2018-06-20
Accepted Date: 2018-05-10
Authors: Kilic, Tugba
Valinhas, Ana Teresa De Sousa
Wall, Ivan ( 0000-0001-6294-8348)
Renaud, Philippe
Carrara, Sandro



Version: Published Version

License: Creative Commons Attribution

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