Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

Khokhlova, Anna, Zolotovskii, Igor, Pogodina, Evgeniia, Saenko, Yuri, Stoliarov, Dmitrii, Vorsina, Svetlana, Fotiadi, Andrei, Liamina, Daria, Sokolovski, Sergei and Rafailov, Edik (2019). Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells. Proceedings of SPIE - International Society for Optical Engineering, 10861 ,

Abstract

The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm2, respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.

Publication DOI: https://doi.org/10.1117/12.2509529
Divisions: Engineering & Applied Sciences > Aston Institute of Photonics Technology
Engineering & Applied Sciences
Additional Information: Copyright 2019 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Uncontrolled Keywords: 1265 nm laser irradiation,DNA damage,cancer cells,near-infrared lasers,oxidative stress,photobiomodulation therapy,reactive oxygen species,singlet oxygen,Electronic, Optical and Magnetic Materials,Atomic and Molecular Physics, and Optics,Biomaterials,Radiology Nuclear Medicine and imaging
Full Text Link:
Related URLs: https://www.spi ... 12.2509529.full (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Conference article
Published Date: 2019-03-07
Accepted Date: 2019-01-01
Authors: Khokhlova, Anna
Zolotovskii, Igor
Pogodina, Evgeniia
Saenko, Yuri
Stoliarov, Dmitrii
Vorsina, Svetlana
Fotiadi, Andrei
Liamina, Daria
Sokolovski, Sergei ( 0000-0001-7445-7204)
Rafailov, Edik ( 0000-0002-4152-0120)

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