Real-time polarimetric biosensing using macroporous alumina membranes

Álvarez, Jesús, Sola, Laura, Platt, Geoff, Cretich, Marina, Swann, Marcus, Chiari, Marcella, Hill, Daniel and Martinez-Pastor, Juan (2013). Real-time polarimetric biosensing using macroporous alumina membranes. Proceedings of SPIE - International Society for Optical Engineering, 8765 ,

Abstract

We report the first demonstration of real-time biosensing in free standing macroporous alumina membranes. The membranes with their 200 nm diameter pores are ideal candidates for biosensing applications where fast response times for small sample volumes are needed as they allow analytes to flow through the pores close to the bioreceptors immobilized on the pores walls. A bulk refractive index sensitivity of 5.2×10-6 refractive index units was obtained from signal responses to different concentrations of NaCl solutions flowing through the pores. Finally, after functionalizing the alumina pore surfaces with an epoxysilane and then spotting it with β-Lactoglobulin protein, the interactions between the β-lactoglobulin and rabbit anti-β-lactoglobulin, as well as the interaction between the rabbit anti-β-lactoglobulin and a secondary antibody anti-rabbit Immunoglobulin G were monitored in real-time.

Publication DOI: https://doi.org/10.1117/12.2017432
Divisions: Engineering & Applied Sciences > Aston Institute of Photonics Technology
Additional Information: Copyright 2013 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Uncontrolled Keywords: Biological sensing and sensors,Form birefringence,Optical sensing and sensors,Polarimetry,Porous alumina,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biomaterials,Radiology Nuclear Medicine and imaging
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.spi ... 7432.full?SSO=1 (Publisher URL)
Published Date: 2013-05-28
Authors: Álvarez, Jesús
Sola, Laura
Platt, Geoff
Cretich, Marina
Swann, Marcus
Chiari, Marcella
Hill, Daniel ( 0000-0003-3510-7783)
Martinez-Pastor, Juan

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