Real time optical immunosensing with flow-through porous alumina membranes

Álvarez, Jesús, Sola, Laura, Cretich, Marina, Swann, Marcus J., Gylfasson, Kristinn B., Volden, Tormod, Chiari, Marcella and Hill, Daniel (2014). Real time optical immunosensing with flow-through porous alumina membranes. Sensors and Actuators, B: Chemical, 202 , pp. 834-839.

Abstract

Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 μL). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, β-lactoglobulin, by spotting. The immuno-assay then proceeded with the binding of the primary and secondary antibody cognates, rabbit anti-β-lactoglobulin and anti-rabbit IgG respectively. Through the use of streptavidin coated quantum dots as refractive index signal enhancers, a noise floor for individual measurements of 3.7 ng/mL (25 pM) was obtained, with an overall statistical, or formal assay LOD of 33.7 ng/mL (225 pM), for total assay time below 1 h.

Publication DOI: https://doi.org/10.1016/j.snb.2014.06.027
Divisions: Engineering & Applied Sciences > Institute of Photonics
Additional Information: © 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: Copolymer,Form birefringence,Optical biosensing,Polarimetry,Porous alumina,Quantum dots,Electronic, Optical and Magnetic Materials,Instrumentation,Condensed Matter Physics,Surfaces, Coatings and Films,Metals and Alloys,Materials Chemistry,Electrical and Electronic Engineering
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.sci ... 7163?via%3Dihub (Publisher URL)
Published Date: 2014-10-31
Authors: Álvarez, Jesús
Sola, Laura
Cretich, Marina
Swann, Marcus J.
Gylfasson, Kristinn B.
Volden, Tormod
Chiari, Marcella
Hill, Daniel ( 0000-0003-3510-7783)

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