Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm formation

Alves, D R, Gaudion, A, Bean, J E, Perez Esteban, P, Arnot, T C, Harper, D R, Kot, W, Hansen, L H, Enright, M C and Jenkins, A Tobias A (2014). Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm formation. Applied and Environmental Microbiology, 80 (21), pp. 6694-703.


Biofilms are major causes of impairment of wound healing and patient morbidity. One of the most common and aggressive wound pathogens is Staphylococcus aureus, displaying a large repertoire of virulence factors and commonly reduced susceptibility to antibiotics, such as the spread of methicillin-resistant S. aureus (MRSA). Bacteriophages are obligate parasites of bacteria. They multiply intracellularly and lyse their bacterial host, releasing their progeny. We isolated a novel phage, DRA88, which has a broad host range among S. aureus bacteria. Morphologically, the phage belongs to the Myoviridae family and comprises a large double-stranded DNA (dsDNA) genome of 141,907 bp. DRA88 was mixed with phage K to produce a high-titer mixture that showed strong lytic activity against a wide range of S. aureus isolates, including representatives of the major international MRSA clones and coagulase-negative Staphylococcus. Its efficacy was assessed both in planktonic cultures and when treating established biofilms produced by three different biofilm-producing S. aureus isolates. A significant reduction of biofilm biomass over 48 h of treatment was recorded in all cases. The phage mixture may form the basis of an effective treatment for infections caused by S. aureus biofilms.

Publication DOI:
Divisions: Life & Health Sciences > Cell & Tissue Biomedical Research
Life & Health Sciences
Additional Information: Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Uncontrolled Keywords: Bacteriolysis,Biofilms,DNA, Viral,Host Specificity,Molecular Sequence Data,Myoviridae,Sequence Analysis, DNA,Staphylococcus Phages,Staphylococcus aureus,Viral Load,Journal Article,Research Support, Non-U.S. Gov't
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Related URLs: https://aem.asm ... tent/80/21/6694 (Publisher URL)
Published Date: 2014-11
Authors: Alves, D R
Gaudion, A
Bean, J E
Perez Esteban, P ( 0000-0001-7084-1065)
Arnot, T C
Harper, D R
Kot, W
Hansen, L H
Enright, M C
Jenkins, A Tobias A



Version: Accepted Version

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