Bioprocessing strategies to enhance the challenging isolation of neuro-regenerative cells from olfactory mucosa

Georgiou, Melanie, Reis, Joana Neves Dos, Wood, Rachael, Esteban, Patricia Perez, Roberton, Victoria, Mason, Chris, Li, Daqing, Li, Ying, Choi, David and Wall, Ivan (2018). Bioprocessing strategies to enhance the challenging isolation of neuro-regenerative cells from olfactory mucosa. Scientific Reports, 8 (1),

Abstract

Olfactory ensheathing cells (OECs) are a promising potential cell therapy to aid regeneration. However, there are significant challenges in isolating and characterizing them. In the current study, we have explored methods to enhance the recovery of cells expressing OEC marker p75NTR from rat mucosa. With the addition of a 24-hour differential adhesion step, the expression of p75NTR was significantly increased to 73 ± 5% and 46 ± 18% on PDL and laminin matrices respectively. Additionally, the introduction of neurotrophic factor NT-3 and the decrease in serum concentration to 2% FBS resulted in enrichment of OECs, with p75NTR at nearly 100% (100 ± 0% and 98 ± 2% on PDL and laminin respectively), and candidate fibroblast marker Thy1.1 decreased to zero. Culturing OECs at physiologically relevant oxygen tension (2–8%) had a negative impact on p75NTR expression and overall cell survival. Regarding cell potency, co-culture of OECs with NG108-15 neurons resulted in more neuronal growth and potential migration at atmospheric oxygen. Moreover, OECs behaved similarly to a Schwann cell line positive control. In conclusion, this work identified key bioprocessing fundamentals that will underpin future development of OEC-based cell therapies for potential use in spinal cord injury repair. However, there is still much work to do to create optimized isolation methods.

Publication DOI: https://doi.org/10.1038/s41598-018-32748-w
Divisions: Life & Health Sciences
Life & Health Sciences > Cell & Tissue Biomedical Research
Additional Information: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Funding: The authors wish to acknowledge financial support from the Biotechnology and Biological Sciences Research Council (BBSRC; grant number BB/K011154/1)
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Related URLs: http://www.natu ... 598-018-32748-w (Publisher URL)
Published Online Date: 2018-12-01
Published Date: 2018-12-01
Authors: Georgiou, Melanie
Reis, Joana Neves Dos
Wood, Rachael
Esteban, Patricia Perez ( 0000-0001-7084-1065)
Roberton, Victoria
Mason, Chris
Li, Daqing
Li, Ying
Choi, David
Wall, Ivan ( 0000-0001-6294-8348)

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