Agonist-induced formation of FGFR1 homodimers and signaling differ among members of the FGF family

Romero-fernandez, Wilber, Borroto-escuela, Dasiel O., Tarakanov, Alexander O., Mudó, Giuseppa, Narvaez, Manuel, Pérez-alea, Mileidys, Agnati, Luigi F., Ciruela, Francisco, Belluardo, Natale and Fuxe, Kjell (2011). Agonist-induced formation of FGFR1 homodimers and signaling differ among members of the FGF family. Biochemical and Biophysical Research Communications, 409 (4), pp. 764-768.


Fibroblast growth factor receptor 1 (FGFR1) is known to be activated by homodimerization in the pres-ence of both the FGF agonist ligand and heparan sulfate glycosaminoglycan. FGFR1 homodimers in turntrigger a variety of downstream signaling cascades via autophosphorylation of tyrosine residues in thecytoplasmic domain of FGFR1. By means of Bioluminescence Energy Resonance Transfer (BRET) as a signof FGFR1 homodimerization, we evaluated in HEK293T cells the effects of all known FGF agonist ligandson homodimer formation. A significant correlation between BRET2signaling and ERK1/2 phosphorylationwas observed, leading to a further characterization of the binding and signaling properties of the FGF sub-families. FGF agonist ligand-FGFR1 binding interactions appear as the main mechanism for the control ofFGFR1 homodimerization and MAPK signaling which demonstrated a high correlation. The bioinformaticanalysis demonstrates the interface of the two pro-triplets SSS (Ser–Ser–Ser) and YGS (Tyr–Gly–Ser)located in the extracellular and intracellular domain of the FGFR1. These pro-triplets are postulated par-ticipate in the FGFR1 homodimerization interface interaction. The findings also reveal that FGF agonistligands within the same subfamily of the FGF gene family produced similar increases in FGFR1 homodi-mer formation and MAPK signaling. Thus, the evolutionary relationship within this gene family appearsto have a distinct functional relevance.

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Divisions: Life & Health Sciences
Full Text Link: https://iris.un ... munications.pdf
Related URLs: http://linkingh ... 006291X11008527 (Publisher URL)
Published Date: 2011-06-01
Authors: Romero-fernandez, Wilber
Borroto-escuela, Dasiel O.
Tarakanov, Alexander O.
Mudó, Giuseppa
Narvaez, Manuel
Pérez-alea, Mileidys
Agnati, Luigi F.
Ciruela, Francisco
Belluardo, Natale
Fuxe, Kjell

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