Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63

Frank, Alexander, Matiolli, Cleverson C, Viana, Américo J C, Hearn, Timothy J, Kusakina, Jelena, Belbin, Fiona E, Wells Newman, David, Yochikawa, Aline, Cano-Ramirez, Dora L, Chembath, Anupama, Cragg-Barber, Kester, Haydon, Michael J, Hotta, Carlos T, Vincentz, Michel, Webb, Alex A R and Dodd, Antony N (2018). Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63. Current Biology, 28 (16), 2597-2606.e6.

Abstract

Synchronization of circadian clocks to the day-night cycle ensures the correct timing of biological events. This entrainment process is essential to ensure that the phase of the circadian oscillator is synchronized with daily events within the environment [1], to permit accurate anticipation of environmental changes [2, 3]. Entrainment in plants requires phase changes in the circadian oscillator, through unidentified pathways, which alter circadian oscillator gene expression in response to light, temperature, and sugars [4, 5, 6]. To determine how circadian clocks respond to metabolic rhythms, we investigated the mechanisms by which sugars adjust the circadian phase in Arabidopsis [5]. We focused upon metabolic regulation because interactions occur between circadian oscillators and metabolism in several experimental systems [5, 7, 8, 9], but the molecular mechanisms are unidentified. Here, we demonstrate that the transcription factor BASIC LEUCINE ZIPPER63 (bZIP63) regulates the circadian oscillator gene PSEUDO RESPONSE REGULATOR7 (PRR7) to change the circadian phase in response to sugars. We find that SnRK1, a sugar-sensing kinase that regulates bZIP63 activity and circadian period [10, 11, 12, 13, 14] is required for sucrose-induced changes in circadian phase. Furthermore, TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1), which synthesizes the signaling sugar trehalose-6-phosphate, is required for circadian phase adjustment in response to sucrose. We demonstrate that daily rhythms of energy availability can entrain the circadian oscillator through the function of bZIP63, TPS1, and the KIN10 subunit of the SnRK1 energy sensor. This identifies a molecular mechanism that adjusts the circadian phase in response to sugars.

Publication DOI: https://doi.org/10.1016/j.cub.2018.05.092
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Additional Information: © 2018 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords: circadian rhythms,signal transduction,metabolism,sugar signaling
Full Text Link:
Related URLs: https://www.sci ... 59a5f9aeaa92ffb (Publisher URL)
Published Online Date: 2018-08-02
Published Date: 2018-08-20
Authors: Frank, Alexander
Matiolli, Cleverson C
Viana, Américo J C
Hearn, Timothy J
Kusakina, Jelena
Belbin, Fiona E
Wells Newman, David
Yochikawa, Aline
Cano-Ramirez, Dora L
Chembath, Anupama ( 0000-0002-3684-2029)
Cragg-Barber, Kester
Haydon, Michael J
Hotta, Carlos T
Vincentz, Michel
Webb, Alex A R
Dodd, Antony N

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