The photobiomodulation of vital parameters of the cancer cell culture by low dose of near-IR laser irradiation

Khokhlova, Anna, Zolotovskii, Igor, Stoliarov, Dmitry, Vorsina, Svetlana, Liamina, Daria, Pogodina, Evgenia Sergeevna, Fotiadi, Andrei, Sokolovski, Sergei G., Saenko, Yury Vladimirovich and Rafailov, Edik U. (2019). The photobiomodulation of vital parameters of the cancer cell culture by low dose of near-IR laser irradiation. IEEE Journal of Selected Topics in Quantum Electronics, 25 (1),

Abstract

The mechanisms underlining the cell adaptive and/or activating oxidative stress, called low level light or photobiomodulation therapies (PBMT), still remain unclear for the near-infrared spectrum range (750-3000 nm), especially for the 1265-1270 nm range (highest absorption by molecular oxygen). It is most probable that the mitochondria may also appear to be the main target for these wavelengths. It is known that mitochondria can generate ROS under visible and 800-1060 nm spectrum range irradiation, which in turn control voltage-dependent anion channels (VDAC). Here we investigated cellular damage regarding VDAC activity, level of oxidative stress, malondialdehyde content, cell viability, mitochondrial potential and mass, GSH level, mitochondrial and nuclear DNA damage in the cancer cell culture exposed to low-level laser irradiation at 1265 nm. We used a continuous wave laser with output power 4 mW; the energy densities employed were 0.3-9.45 J/cm 2. We observed that the laser radiation at 1265 nm can induce the oxidative stress, enhance apoptosis, and disturb mitochondrial functioning at the energy density of 9.54 J/cm 2. In addition, inhibition of VDAC enhances the observed effects. It has been shown that the laser irradiation at 1265 nm damages mitochondrial DNA but does not affect the nuclear DNA. The performed experiments bring us to the conclusion that the laser irradiation at 1265 nm can affect cells through mitochondrial damage and the inhibition of VDAC enhances effects of PBMT.

Publication DOI: https://doi.org/10.1109/JSTQE.2018.2854539
Divisions: Engineering & Applied Sciences > Aston Institute of Photonics Technology
Engineering & Applied Sciences
Additional Information: © 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Uncontrolled Keywords: Biophotonics,cancer cell,near-infrared lasers,photobiomodulation therapy,Atomic and Molecular Physics, and Optics,Electrical and Electronic Engineering
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Related URLs: https://ieeexpl ... cument/8410463/ (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2019-01-01
Published Online Date: 2018-07-12
Accepted Date: 2018-07-01
Authors: Khokhlova, Anna
Zolotovskii, Igor
Stoliarov, Dmitry
Vorsina, Svetlana
Liamina, Daria
Pogodina, Evgenia Sergeevna
Fotiadi, Andrei
Sokolovski, Sergei G. ( 0000-0001-7445-7204)
Saenko, Yury Vladimirovich
Rafailov, Edik U. ( 0000-0002-4152-0120)

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