Technical challenges of working with extracellular vesicles

Ramirez, Marcel I., Amorim, Maria G., Gadelha, Catarina, Milic, Ivana, Welsh, Joshua A., Freitas, Vanessa M., Nawaz, Muhammad, Akbar, Naveed, Couch, Yvonne, Makin, Laura, Cooke, Fiona, Vettore, Andre L., Batista, Patricia X., Freezor, Roberta, Pezuk, Julia A., Rosa-Fernandes, Lívia, Carreira, Ana Claudia O., Devitt, Andrew, Jacobs, Laura, Silva, Israel T., Coakley, Gillian, Nunes, Diana N., Carter, Dave, Palmisano, Giuseppe and Dias-Neto, Emmanuel (2017). Technical challenges of working with extracellular vesicles. Nanoscale, 10 (3), pp. 881-906.

Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Publication DOI: https://doi.org/10.1039/c7nr08360b
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Life & Health Sciences > Cell & Tissue Biomedical Research
Life & Health Sciences > Chronic and Communicable Conditions
Life & Health Sciences > Cellular and Molecular Biomedicine
Additional Information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Uncontrolled Keywords: Materials Science(all)
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://pubs.rsc ... 0b#!divAbstract (Publisher URL)
Published Date: 2017-12-13
Authors: Ramirez, Marcel I.
Amorim, Maria G.
Gadelha, Catarina
Milic, Ivana ( 0000-0001-7531-7561)
Welsh, Joshua A.
Freitas, Vanessa M.
Nawaz, Muhammad
Akbar, Naveed
Couch, Yvonne
Makin, Laura
Cooke, Fiona
Vettore, Andre L.
Batista, Patricia X.
Freezor, Roberta
Pezuk, Julia A.
Rosa-Fernandes, Lívia
Carreira, Ana Claudia O.
Devitt, Andrew ( 0000-0002-4651-6761)
Jacobs, Laura
Silva, Israel T.
Coakley, Gillian
Nunes, Diana N.
Carter, Dave
Palmisano, Giuseppe
Dias-Neto, Emmanuel

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