An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography

Abstract

This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

Publication DOI: https://doi.org/10.1039/C7LC00812K
Divisions: Engineering & Applied Sciences > Chemical Engineering & Applied Chemistry
Engineering & Applied Sciences
Engineering & Applied Sciences > Mechanical Engineering & Design
Engineering & Applied Sciences > Aston Institute of Materials Research (AIMR)
Additional Information: © Royal Society of Chemistry 2017 Funding: Strategic Award from the Wellcome Trust and the Biotechnology and Biological Sciences Research Council (grant 102593).
Full Text Link:
Related URLs: http://pubs.rsc ... 7/LC/C7LC00812K (Publisher URL)
PURE Output Type: Article
Published Date: 2017-11-01
Accepted Date: 2017-11-01
Authors: Burton, Christian George George
Axford, Danny
Edwards, Andrew Michael John
Gildea, Richard J
Morris, Robert H
Newton, Michael I
Orville, Allen M
Prince, Mark ( 0000-0002-3709-099X)
Topham, Paul ( 0000-0003-4152-6976)
Docker, Peter

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