A new panel of epitope mapped monoclonal antibodies recognising the prototypical tetraspanin CD81

Grove, Joe, Hu, Ke, Farquhar, Michelle J., Goodall, Margaret, Walker, Lucas, Jamshad, Mohammed, Drummer, Heidi E., Bill, Roslyn M., Balfe, Peter and McKeating, Jane A. (2017). A new panel of epitope mapped monoclonal antibodies recognising the prototypical tetraspanin CD81. Wellcome Open Research, 2 , p. 82.

Abstract

Background: Tetraspanins are small transmembrane proteins, found in all higher eukaryotes, that compartmentalize cellular membranes through interactions with partner proteins. CD81 is a prototypical tetraspanin and contributes to numerous physiological and pathological processes, including acting as a critical entry receptor for hepatitis C virus (HCV). Antibody engagement of tetraspanins can induce a variety of effects, including actin cytoskeletal rearrangements, activation of MAPK-ERK signaling and cell migration. However, the epitope specificity of most anti-tetraspanin antibodies is not known, limiting mechanistic interpretation of these studies. Methods: We generated a panel of monoclonal antibodies (mAbs) specific for CD81 second extracellular domain (EC2) and performed detailed epitope mapping with a panel of CD81 mutants. All mAbs were screened for their ability to inhibit HCV infection and E2-CD81 association. Nanoscale distribution of cell surface CD81 was investigated by scanning electron microscopy. Results: The antibodies were classified in two epitope groups targeting opposing sides of EC2. We observed a wide range of anti-HCV potencies that were independent of their epitope grouping, but associated with their relative affinity for cell-surface expressed CD81. Scanning electron microscopy identified at least two populations of CD81; monodisperse and higher-order assemblies, consistent with tetraspanin-enriched microdomains. Conclusions: These novel antibodies provide well-characterised tools to investigate CD81 function, including HCV entry, and have the potential to provide insights into tetraspanin biology in general.

Publication DOI: https://doi.org/10.12688/wellcomeopenres.12058.1
Divisions: Life & Health Sciences
Life & Health Sciences > Cellular and Molecular Biomedicine
Life & Health Sciences > Biosciences
Additional Information: Funding: BBSRC (BB/N007417/1); EC (LSHG-CT-2004-504601 and FP7 F3-2012-305578); MRC (MC_UU_12018/1 and G1100247); Wellcome Trust (200838); and Royal Society (107653).
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Related URLs: https://www.sco ... a19ec1cc9a66a1c (Scopus URL)
https://wellcom ... rticles/2-82/v1 (Publisher URL)
Published Date: 2017-09-07
Authors: Grove, Joe
Hu, Ke
Farquhar, Michelle J.
Goodall, Margaret
Walker, Lucas
Jamshad, Mohammed
Drummer, Heidi E.
Bill, Roslyn M. ( 0000-0003-1331-0852)
Balfe, Peter
McKeating, Jane A.

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Version: Accepted Version

License: Creative Commons Attribution

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