Human heart failure biomarker immunosensor based on excessively tilted fiber gratings

Luo, Binbin, Wu, Shengxi, Zhang, Zhonghao, Zou, Wengen, Shi, Shenghui, Zhao, Mingfu, Zhong, Nianbing, Liu, Yong, Zou, Xue, Wang, Lingling, Chai, Weina, Hu, Chuanmin and Zhang, Lin (2017). Human heart failure biomarker immunosensor based on excessively tilted fiber gratings. Biomedical Optics Express, 8 (1), pp. 57-67.


A label-free immunosensor platform based on excessively tilted fiber gratings (Ex- TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NTproBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NTproBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.

Publication DOI:
Divisions: Engineering & Applied Sciences > Electrical, Electronic & Power Engineering
Engineering & Applied Sciences > Aston Institute of Photonics Technology
Additional Information: © 2016 Optical Society of America
Uncontrolled Keywords: biological sensing and sensors,fiber optics sensors,medical optics and biotechnology,Biotechnology,Atomic and Molecular Physics, and Optics
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2017-01-01
Published Online Date: 2016-12-05
Accepted Date: 2016-11-30
Submitted Date: 2016-08-01
Authors: Luo, Binbin
Wu, Shengxi
Zhang, Zhonghao
Zou, Wengen
Shi, Shenghui
Zhao, Mingfu
Zhong, Nianbing
Liu, Yong
Zou, Xue
Wang, Lingling
Chai, Weina
Hu, Chuanmin
Zhang, Lin ( 0000-0002-1691-5577)



Version: Published Version

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