The development of attenuation compensation models of fluorescence spectroscopy signals


This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry. We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra. In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.

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Divisions: Engineering & Applied Sciences > Electrical, Electronic & Power Engineering
Engineering & Applied Sciences
Additional Information: -Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova and Andrey V. Dunaev, "The development of attenuation compensation models of fluorescence spectroscopy signals", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (April 21, 2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI:
Event Title: 3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015
Event Type: Other
Event Dates: 2015-09-22 - 2015-09-25
Uncontrolled Keywords: 3D model,correction spectra,diffuse reflectance spectroscopy,fluorescence spectroscopy,laser Doppler flowmetry,Monte Carlo simulation,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biomaterials,Radiology Nuclear Medicine and imaging
ISBN: 978-1-5106-0226-7
Full Text Link: http://proceedi ... ticleid=2516953
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Conference contribution
Published Date: 2016-04-21
Accepted Date: 2016-03-31
Authors: Dremin, Victor V.
Zherebtsov, Evgeny A.
Rafailov, Ilya E.
Vinokurov, Andrey Y.
Novikova, Irina N.
Zherebtsova, Angelina I.
Litvinova, Karina S. ( 0000-0003-1110-8589)
Dunaev, Andrey V.



Version: Published Version

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