Anticancer effects of Curcuma C20-dialdehyde against colon and cervical cancer cell lines

Chaithongyot, Supattra, Asgar, Ali, Senawong, Gulsiri, Yowapuy, Anongnat, Lattmann, Eric, Sattayasai, Nison and Senawong, Thanaset (2015). Anticancer effects of Curcuma C20-dialdehyde against colon and cervical cancer cell lines. Asian Pacific Journal of Cancer Prevention, 16 (15), pp. 6513-6519.

Abstract

Background: Recent attention on chemotherapeutic intervention against cancer has been focused on discovering and developing phytochemicals as anticancer agents with improved efficacy, low drug resistance and toxicity, low cost and limited adverse side effects. In this study, we investigated the effects of Curcuma C20-dialdehyde on growth, apoptosis and cell cycle arrest in colon and cervical cancer cell lines. Materials and Methods: Antiproliferative, apoptosis induction, and cell cycle arrest activities of Curcuma C20-dialdehyde were determined by WST cell proliferation assay, flow cytometric Alexa fluor 488-annexin V/propidium iodide (PI) staining and PI staining, respectively. Results: Curcuma C20 dialdehyde suppressed the proliferation of HCT116, HT29 and HeLa cells, with IC50 values of 65.4±1.74 μg/ml, 58.4±5.20 μg/ml and 72.0±0.03 μg/ml, respectively, with 72 h exposure. Flow cytometric analysis revealed that percentages of early apoptotic cells increased in a dose-dependent manner upon exposure to Curcuma C20-dialdehyde. Furthermore, exposure to lower concentrations of this compound significantly induced cell cycle arrest at G1 phase for both HCT116 and HT29 cells, while higher concentrations increased sub-G1 populations. However, the concentrations used in this study could not induce cell cycle arrest but rather induced apoptotic cell death in HeLa cells. Conclusions: Our findings suggest that the phytochemical Curcuma C20-dialdehyde may be a potential antineoplastic agent for colon and cervical cancer chemotherapy and/or chemoprevention. Further studies are needed to characterize the drug target or mode of action of the Curcuma C20-dialdehyde as an anticancer agent.

Publication DOI: https://doi.org/10.7314/APJCP.2015.16.15.6513
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Additional Information: Published and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/ licenses/by/3.0/), under which authors reserve the copyright for their content; however, they permit anyone unrestricted non-commercial use as long as the original authors and source are cited.
Uncontrolled Keywords: antiproliferative activity,apoptosis,cell cycle arrest,cervical,colon cancer,curcuma C20-dialdehyde,Oncology,Cancer Research,Public Health, Environmental and Occupational Health,Epidemiology
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2015-10-06
Authors: Chaithongyot, Supattra
Asgar, Ali
Senawong, Gulsiri
Yowapuy, Anongnat
Lattmann, Eric
Sattayasai, Nison
Senawong, Thanaset

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