Effect of nitric oxide on apoptotic activity in the rat gastrointestinal tract

Bersimbaev, Rakhmet I., Yugai, Yelena E., Hanson, Peter J. and Tzoy, Igor G. (2001). Effect of nitric oxide on apoptotic activity in the rat gastrointestinal tract. European Journal of Pharmacology, 423 (1), pp. 9-16.

Abstract

The effect of nitric oxide (NO) on apoptosis in the gastrointestinal mucosa was investigated. Experiments involved long-term exposure of rat gastric mucosal cells in vitro to exogenous NO delivered from the NO, donor S-nitroso-N-acetyl-penicillamine, and the effect of intravenous administration of lipopolysaccharide in vivo, in the presence and absence of the selective inhibitor of inducible NO synthase N-(3-(aminomethyl)benzyl) acetamidine (1400 W). S-nitroso-N-acetyl-penicillamine produced a dose-related inhibition of caspase 3-like activity and DNA fragmentation in isolated gastric mucosal cells. Caspase 3-like activity and DNA fragmentation in gastric, ileal and colonic mucosa were increased both 5 and 24 h after injection of lipopolysaccharide (3 mg/kg, i.v.) to rats in vivo. Administration of 1400 W (5 mg/kg, i.v.) immediately after lipopolysaccharide enhanced caspase 3-like activity and DNA fragmentation above that found with lipopolysaccharide alone. In conclusion, data obtained both in vitro and in vivo suggest that NO exerts an anti-apoptotic effect on rat gastrointestinal mucosal cells. © 2001 Elsevier Science B.V.

Publication DOI: https://doi.org/10.1016/S0014-2999(01)01095-0
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Uncontrolled Keywords: nitric oxide (NO),apoptosis,gastrointestinal tract,lipopolysaccharide,Cellular and Molecular Neuroscience,Pharmacology
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2001-06-29
Authors: Bersimbaev, Rakhmet I.
Yugai, Yelena E.
Hanson, Peter J. ( 0000-0001-6905-6051)
Tzoy, Igor G.

Download

Item under embargo.

Export / Share Citation


Statistics

Additional statistics for this record