Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells

Dever, Gary, Stewart, Laura-Jayne, Pitt, Andrew and Spickett, Corinne M. (2003). Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells. FEBS Letters, 540 (1-3), pp. 245-250.

Abstract

Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (111,60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10100 muM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.

Publication DOI: https://doi.org/10.1016/S0014-5793(03)00271-0
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Life & Health Sciences > Cellular and Molecular Biomedicine
Life & Health Sciences > Biosciences
Life & Health Sciences > Chronic and Communicable Conditions
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Uncontrolled Keywords: phosphatidylcholine,HOCl,oxidative stress,chlorohydrin,HL60,atherosclerosis,Pharmacy and materia medica
Published Date: 2003-04-10
Authors: Dever, Gary
Stewart, Laura-Jayne
Pitt, Andrew ( 0000-0003-3619-6503)
Spickett, Corinne M. ( 0000-0003-4054-9279)

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