Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment

Sandison, Mairi E., Tveen Jensen, K., Gesellchen, F., Cooper, J.M. and Pitt, A.R. (2014). Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment. Analyst, 139 (19), pp. 4974-4981.

Abstract

Reversible phosphorylation plays a key role in numerous biological processes. Mass spectrometry-based approaches are commonly used to analyze protein phosphorylation, but such analysis is challenging, largely due to the low phosphorylation stoichiometry. Hence, a number of phosphopeptide enrichment strategies have been developed, including metal oxide affinity chromatography (MOAC). Here, we describe a new material for performing MOAC that employs a magnetite-doped polydimethylsiloxane (PDMS), that is suitable for the creation of microwell array and microfluidic systems to enable low volume, high throughput analysis. Incubation time and sample loading were explored and optimized and demonstrate that the embedded magnetite is able to enrich phosphopeptides. This substrate-based approach is rapid, straightforward and suitable for simultaneously performing multiple, low volume enrichments.

Publication DOI: https://doi.org/10.1039/C4AN00750F
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Life & Health Sciences > Cellular and Molecular Biomedicine
Additional Information: Funding: RASOR (“Radical Solutions for Researching the proteome”) BBSRC IRC programme (BB/C511572/1) and the Proxomics EPSRC Cross-Disciplinary Research Landscape Award (EP/I017887/1). Electronic supplementary information: DOI 10.1039/c4an00750f
Uncontrolled Keywords: Analytical Chemistry,Spectroscopy,Electrochemistry,Biochemistry,Environmental Chemistry
Full Text Link: http://pubs.rsc ... 4/an/c4an00750f
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2014-10-07
Authors: Sandison, Mairi E.
Tveen Jensen, K.
Gesellchen, F.
Cooper, J.M.
Pitt, A.R. ( 0000-0003-3619-6503)

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License: Creative Commons Attribution


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