Novel expression of microRNAs in serum samples of Iraqi breast cancer women

Saad, Zaynab, Arif, Muhammad, Yassen, Nahi, Jasim, Hameed, Jelawe, Majed and Brown, James (2014). Novel expression of microRNAs in serum samples of Iraqi breast cancer women. American Journal of Biomedicine, 2 (5), pp. 567-574.

Abstract

Although a lot of hard work against cancer to reduces its spread but it still continues to kill with abandon. The need for a biomarker for cancer early detection becomes the most mind concentrated scientists. MicroRNAs the tiny non coding RNA molecules opened new path for the scientists to determine the cancer in its early stages. Expression of microRNAs profiles has been investigated to be involved in cancer development. Here we determined the expression of microRNAs in serum of Iraqi healthy volunteers and other women diagnosed with breast cancer. MicroRNAs expression has been determined by using real time qPCR and delta method has been used. Four of thirteen microRNAs were shown to be expressed in serum of Iraqi breast cancer women. Let-7a and miR-21 were shown to be significantly over expressed in serum of breast cancer compared with healthy serum volunteers (P= 0.022 and 0.026) respectively. While miR-26b and miR-429 found to be significantly down expressed in serum of breast cancer women (P= 0.0034 and 0.031) respectively. The result concluded that these expressed microRNAs in serum of breast cancer women could be used as a first indicator of breast cancer occurrence.

Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Additional Information: Copyright © 2014 ZS. et al. This is article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: microRNA,breast cancer,reverse transcription,real time PCR
Full Text Link: http://ajbm.net ... t-cancer-women/
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Published Date: 2014-05-01
Authors: Saad, Zaynab
Arif, Muhammad
Yassen, Nahi
Jasim, Hameed
Jelawe, Majed
Brown, James ( 0000-0002-3504-7373)

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