A simple bioanalytical method for the quantification of antiepileptic drugs in dried blood spots


An increasing number of publications on the dried blood spot (DBS) sampling approach for the quantification of drugs and metabolites have been spurred on by the inherent advantages of this sampling technique. In the present research, a selective and sensitive high-performance liquid chromatography method for the concurrent determination of multiple antiepileptic drugs (AEDs) [levetiracetam (LVT), lamotrigine (LTG), phenobarbital (PHB)], carbamazepine (CBZ) and its active metabolite carbamazepine-10,11 epoxide (CBZE)] in a single DBS has been developed and validated. Whole blood was spotted onto Guthrie cards and dried. Using a standard punch (6 mm diameter), a circular disc was punched from the card and extracted with methanol: acetonitrile (3:1, v/v) containing hexobarbital (Internal Standard) and sonicated prior to evaporation. The extract was then dissolved in water and vortex mixed before undergoing solid phase extraction using HLB cartridges. Chromatographic separation of the AEDs was achieved using Waters XBridge™ C18 column with a gradient system. The developed method was linear over the concentration ranges studied with r ≥ 0.995 for all compounds. The lower limits of quantification (LLOQs) were 2, 1, 2, 0.5 and 1 μg/mL for LVT, LTG, PHB, CBZE and CBZ, respectively. Accuracy (%RE) and precision (%CV) values for within and between day were <20% at the LLOQs and <15% at all other concentrations tested. This method was successfully applied to the analysis of the AEDs in DBS samples taken from children with epilepsy for the assessment of their adherence to prescribed treatments.

Publication DOI: https://doi.org/10.1016/j.jchromb.2013.02.005
Divisions: Life & Health Sciences > Pharmacy
Additional Information: Copyright © 2013 Elsevier B.V. All rights reserved.
Uncontrolled Keywords: antiepileptic drugs,dried blood spot,HPLC,children
Full Text Link: https://pure.qu ... blood_spots.pdf
Related URLs: https://www.sci ... 0901?via%3Dihub (Publisher URL)
https://pure.qu ... blood_spots.pdf (Organisation URL)
PURE Output Type: Article
Published Date: 2013-04-01
Authors: Shah, N. Mohamed
Hawwa, A.F.
Millership, J.S.
Collier, P.S.
McElnay, J.C.

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