Active membrane transport and receptor proteins from bacteria


A general strategy for the expression of bacterial membrane transport and receptor genes in Escherichia coli is described. Expression is amplified so that the encoded proteins comprise 5-35% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGSH6 or a Strep tag at the C-terminus. These enable purification in mg quantities for crystallization and NMR studies. Examples of one nutrient uptake and one multidrug extrusion protein from Helicobacter pylori are described. This strategy is successful for membrane proteins from H. pylori, E. coli, Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Microbacterium liquefaciens, Brucella abortus, Brucella melitensis, Campylobacter jejuni, Neisseria meningitides, Streptomyces coelicolor and Rhodobacter sphaeroides. ©2005 Biochemical Society.

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Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Cellular and Molecular Biomedicine
Additional Information: Copyright 2005 The Biochemical Society. The Accepted Manuscript is reproduced here in accordance with the copyright policy of the publisher. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from Portland Press Limited. The final Version of Record is available at Conference on Mechanisms of Bioenergetic Membrane Proteins - Structures and Beyond, Wiesbaden (DE), 20-24 March 2005
Uncontrolled Keywords: bacteria,helicobacter pylori,his tag,membrane transport protein,pathogen,two-component system,Biochemistry
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://www.bioc ... /bst0330867.htm (Publisher URL)
Published Date: 2005-08
Authors: Saidijam, M.
Bettaney, K.E.
Szakonyi, G.
Psakis, G.
Shibayama, K.
Suzuki, S.
Clough, J.L.
Blessie, V.
Abu-bakr, A.
Baumberg, S.
Meuller, J.
Hoyle, C.K.
Palmer, S.L.
Butaye, P.
Walravens, K.
Patching, S.G.
O'Reilly, J.
Rutherford, NG.
Bill, Roslyn M. ( 0000-0003-1331-0852)
Roper, D.I.
Phillips-Jones, M.K.
Henderson, P.J.F.



Version: Accepted Version

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