A sequential mechanism for clathrin cage disassembly by 70-kDa heat-shock cognate protein (Hsc70) and auxilin

Rothnie, Alice, Clarke, Anthony R., Kuzmic, Petr, Cameron, Angus and Smith, Corinne J. (2011). A sequential mechanism for clathrin cage disassembly by 70-kDa heat-shock cognate protein (Hsc70) and auxilin. Proceedings of the National Academy of Sciences, 108 (17), pp. 6927-6932.

Abstract

An essential stage in endocytic coated vesicle recycling is the dissociation of clathrin from the vesicle coat by the molecular chaperone, 70-kDa heat-shock cognate protein (Hsc70), and the J-domain-containing protein, auxilin, in an ATP-dependent process. We present a detailed mechanistic analysis of clathrin disassembly catalyzed by Hsc70 and auxilin, using loss of perpendicular light scattering to monitor the process. We report that a single auxilin per clathrin triskelion is required for maximal rate of disassembly, that ATP is hydrolyzed at the same rate that disassembly occurs, and that three ATP molecules are hydrolyzed per clathrin triskelion released. Stopped-flow measurements revealed a lag phase in which the scattering intensity increased owing to association of Hsc70 with clathrin cages followed by serial rounds of ATP hydrolysis prior to triskelion removal. Global fit of stopped-flow data to several physically plausible mechanisms showed the best fit to a model in which sequential hydrolysis of three separate ATP molecules is required for the eventual release of a triskelion from the clathrin-auxilin cage.

Publication DOI: https://doi.org/10.1073/pnas.1018845108
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Cellular and Molecular Biomedicine
Uncontrolled Keywords: clathrin,Hsc70,auxilin,light scattering,stopped-flow,kKinetics,molecular models,multiprotein complexes,clathrin-mediated endocytosis,kinetic mechanism,macromolecular assemblies,vesicle uncoating,mathematical model,General
Full Text Link: http://www.pnas ... ent/108/17/6927
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2011-04-26
Authors: Rothnie, Alice ( 0000-0002-4259-7015)
Clarke, Anthony R.
Kuzmic, Petr
Cameron, Angus
Smith, Corinne J.

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