An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples


Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.

Publication DOI:
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Chronic and Communicable Conditions
Uncontrolled Keywords: aldehydes,high pressure liquid chromatography,liquid chromatography,clinical chemistry tTests,enzyme-linked immunosorbent assay,humans,isoprostanes,lipid peroxidation,malondialdehyde,mass spectrometry,plasma,reproducibility of results,sensitivity and specificity,ultraviolet rays,oxidative stress,4-hydroxynonenal,F2-isoprostanes,Biochemistry
Full Text Link: https://iris.un ... %20BREUSING.pdf
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2010-10
Authors: Breusing, Nicolle
Grune, Tilman
Andrisic, Luka
Atalay, Mustafa
Bartosz, Grzegorz
Biasi, Fiorella
Borovic, Suzana
Bravo, Laura
Casals, Isidre
Casillas, Rosario
Dinischiotu, Anca
Drzewinska, Joanna
Faber, Heidemarie
Fauzi, Norsyahida M.
Gajewska, Agnieszka
Gambini, Juan
Gradinaru, Daniela
Kokkola, Tarja
Lojek, Antonin
Łuczaj, Wojciech
Margina, Denisa
Mascia, Cinzia
Mateos, Raquel
Meinitzer, Andreas
Mitjavila, María T.
Mrakovcic, Lidija
Munteanu, Maria C.
Podborska, Martina
Poli, Giuseppe
Sicinska, Paulina
Skrzydlewska, Elzbieta
Vina, Jose
Wiswedel, Ingrid
Zarkovic, Neven
Zelzer, Sieglinde
Spickett, Corinne M ( 0000-0003-4054-9279)

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