The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network

Wassmer, Thomas, Attar, Naomi, Harterink, Martin, van Weering, Jan R.T., Traer, Colin J., Oakley, Jacqueline, Goud, Bruno, Stephens, David J., Verkade, Paul, Korswagen, Hendrik C. and Cullen, Peter J. (2009). The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network. Developmental Cell, 17 (1), pp. 110-122.

Abstract

Early endosome-to-trans-Golgi network (TGN) transport is organized by the retromer complex. Consisting of cargo-selective and membrane-bound subcomplexes, retromer coordinates sorting with membrane deformation and carrier formation. Here, we describe four mammalian retromers whose membrane-bound subcomplexes contain specific combinations of the sorting nexins (SNX), SNX1, SNX2, SNX5, and SNX6. We establish that retromer requires a dynamic spatial organization of the endosomal network, which is regulated through association of SNX5/SNX6 with the p150(glued) component of dynactin, an activator of the minus-end directed microtubule motor dynein; an association further defined through genetic studies in C. elegans. Finally, we also establish that the spatial organization of the retromer pathway is mediated through the association of SNX1 with the proposed TGN-localized tether Rab6-interacting protein-1. These interactions describe fundamental steps in retromer-mediated transport and establish that the spatial organization of the retromer network is a critical element required for efficient retromer-mediated sorting.

Publication DOI: https://doi.org/10.1016/j.devcel.2009.04.016
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Cellular and Molecular Biomedicine
Additional Information: Open access under CC BY license
Uncontrolled Keywords: animals,biological transport,caenorhabditis elegans,caenorhabditis elegans proteins,carrier proteins,cell line,dyneins,endosomes,humans,microtubule-associated proteins,multiprotein complexes,phylogeny,protein isoforms,RNA interference,recombinant fusion proteins,sorting nexins,two-hybrid system techniques,vesicular transport proteins,rab GTP-binding proteins,trans-Golgi network,Developmental Biology
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2009-07-21
Authors: Wassmer, Thomas
Attar, Naomi
Harterink, Martin
van Weering, Jan R.T.
Traer, Colin J.
Oakley, Jacqueline
Goud, Bruno
Stephens, David J.
Verkade, Paul
Korswagen, Hendrik C.
Cullen, Peter J.

Download

[img]

Version: Published Version

License: Creative Commons Attribution

| Preview

Export / Share Citation


Statistics

Additional statistics for this record