Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry


Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.

Publication DOI:
Divisions: Life & Health Sciences
Life & Health Sciences > Pharmacy
Life & Health Sciences > Cellular and Molecular Biomedicine
Additional Information: Copyright © 2011 John Wiley & Sons, Ltd.
Uncontrolled Keywords: animals,brain chemistry,Fourier analysis,histocytochemistry,kidney,metabolomics,mice,phospholipids,spectrometry,tin compounds,mass,matrix-assisted laser desorption-ionization,Spectroscopy,Analytical Chemistry,Organic Chemistry
Full Text Link: https://www.ncb ... les/PMC3505379/
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://onlineli ... m.4939/abstract (Publisher URL)
PURE Output Type: Article
Published Date: 2011-04-15
Published Online Date: 2011-03-14
Authors: Goodwin, Richard J.A.
Pitt, Andrew R. ( 0000-0003-3619-6503)
Harrison, David
Weidt, Stefan K.
Langridge-Smith, Pat R.R.
Barrett, Michael P.
Mackay, C. Logan

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